Library Prep & Pooling Calculator

Calculate Molarity (nM)

Concentration (ng/µL)

Average Library Size (bp)

Initial Concentration (nM)

Desired Final Concentration (nM)

Target Concentration (ng/µL)

Final Volume (µL)

Calculates volumes to create a final mix with a target concentration and spike-in ratio.

Library Stock Conc.

Spike-in Stock Conc.

Desired Final Conc.

Final Volume (µL)

Spike-in Percentage (%)

Calculates the volume of spike-in to add to a pre-existing library solution.

Library Conc.

Library Volume (µL)

Spike-in Stock Conc.

Spike-in Percentage (%)